RESOURCE Q columns are prepacked with SOURCE 15Q, a strong anion exchanger for high resolution polishing purification of proteins at high flow rates. Quaternary ammonium (Q) strong anion exchanger for high resolution ion exchange chromatography. Mono-sized 15 µm rigid beads give low back pressure and high flow rates allowing for high productivity.
RESOURCE Q, 1 mL and 6 mL RESOURCE S, 1 mL and 6 mL Ion exchange columns Instructions for Use RESOURCE™ Q and S are prepacked colu mns for separating biomolecules by ion exchange chromatography. The columns are prepacked with SOURCE™ 15Q or SOURCE 15S. The resin is based on rigid, monodisperse ~ 15 ?m beads made of polystyrene/divinyl.
RESOURCE ™ Q columns are prepacked with SOURCE ™ 15Q, a strong anion exchanger for intermediate lab-scale purification and large-scale polishing of biomolecules. The small (15 ?m) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high …
To obtain high-purity FAU-1 proteins, these elution fractions were further purified with RESOURCE-Q anion-exchange column chromatography (GE Healthcare, Piscataway, NJ, USA) with a linear gradient of 0–1 M NaCl in Tris-HCl buffer (50 mM Tris-HCl [pH 8.0], 1 mM EDTA, 0.02% Tween 20, 7 mM 2-mercaptoethanol, 10% glycerol). .. The collected fractions.
Resource 15 Q (GE Healthcare Life Sciences) is an anion – exchange resin used for purification of proteins and peptides. The resin is positively charged so the proteins with negative charge bind to it which can then be eluted by a linear or non-linear gradient of salt.
RESOURCE Q : Quantity: 1 x 6mL: Item Description: RESOURCE Q columns are prepacked with SOURCE 15Q, a strong anion exchanger for high resolution polishing purification of proteins at high flow rates. Diameter (Metric) Inner Column : 16 mm: Flow Rate >60 mL/min. pH Range: 2-12: Ligand Type: Quaternary ammonium, 15 µm Q Strong anion 2 to 12 BSA 45 mg/mL resin RESOURCE PEEK column RESOURCE Q 1 mL 1 mL 6.4 × 30 1 to 10 mL/min 1.5 1 column 17117701 RESOURCE Q 6 mL 6 mL 16 × 30 1 to 60 mL/min 0.6 1 column 17117901 Tricorn PEEK column SOURCE 15 Q 4.6/100 PE 1.7 mL 4.6 × 100 0.5 to 2.5 mL/min 4 1 column 17518101 Resin in bulk SOURCE 15 Q 50 mL N/A.
The dynamic binding capacity of the Resource Q column is 40-54 mg BSA/ml as per the data sheet of GE health care and one can load 25 mg protein/ml to this strong anion exchange column.
Select Anion Exchange at System pH Above pI System pH pH Net Char otein Isoelectric poin t (pI) 0 … RESOURCE Q , 1 ml 010 20min 30 A 280 010 20min 30 Mono Q ( column 5 × 50 mm) A 280 5.0 10.0 15.0ml Mini Q ( column 4.6 × 50 mm) A 280 Sepharose Big Beads ( Q or SP) Sepharose XL ( Q or SP) Capto ( Q , S, MMC) Sepharose Fast Flow ( Q , SP, DEAE, CM, ANX)
